Role of IL-27 in mouse colitis model and its influence on NLRP3 inflam-masome
10.3969/j.issn.1000-4718.2018.06.021
- VernacularTitle:IL-27在小鼠结肠炎中的作用及对NLRP3炎性小体的影响
- Author:
Mu-Han ZHANG
1
;
Li-Wei ZHOU
;
Xin HE
;
Yao LU
;
Mei-Hua ZHAO
;
Na MA
;
Bai-Sui FENG
Author Information
1. 郑州大学第二附属医院消化内科
- Keywords:
Interleukin-27;
NLRP3 inflammasome;
Inflammatory bowel diseases;
Colitis
- From:
Chinese Journal of Pathophysiology
2018;34(6):1089-1094
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of interleukin-27 (IL-27) on the pathological changes and the expres-sion and activation of NOD-like receptor protein 3 (NLRP3) inflammasome in the colonic tissues of the mice with dextran sodium sulfate (DSS)-induced experimental colitis. METHODS:Male C57BL/6 mice (n=48) were randomly divided into control group (given unrestricted diet), DSS group (drinking 3% DSS solution), IL-27 (500 ng) group and IL-27 (1 μg) group (intraperitoneal injection of 500 ng and 1 μg IL-27 on the basis of drinking DSS solution, respectively). After treatment for 12 d, intestinal inflammation in the mice was evaluated, the pathological changes of the colonic tissues were observed by HE staining, and the disease activity index ( DAI) score and histological index ( HI) score were calculated. The colonic tissues were collected for immunohistochemistry, qPCR and Western blot detections. The serum was prepared for ELISA. RESULTS:Compared with control group, the DAI score and HI score in model group indicated that the colo-nic inflammation was more obvious (P<0.05), the mRNA expression of NLRP3 and IL-1β was increased, the protein levels of NLRP3 and cleaved caspase-1 were elevated, and the releases of IL-1β and IL-18 in the serum were also increased (P<0.05). Compared with DSS group, the DAI score and HI score in IL-27 (1 μg) group indicated that the colonic in-flammation was obviously attenuated, the mRNA expression of NLRP3 and IL-1β was decreased, the protein levels of NLRP3 and cleaved caspase-1 were suppressed, and the releases of IL-1β 和 IL-18 in the serum were also decreased (P<0.05). No difference of the above indexes between DSS group and IL-27 (500 ng) group was observed except the de-creases in the releases of IL-1β and IL-18 in the serum in IL-27 (500 ng) group. CONCLUSION:IL-27 alleviates the inflammation in DSS-induced colitis mice and inhibits the expression and activation of NLRP3 inflammasome.
