Renal protective and anti-apoptotic functions of erythropoietin in ze-brafish embryonic development
10.3969/j.issn.1000-4718.2018.06.018
- VernacularTitle:促红细胞生成素在斑马鱼胚胎发育中抑制肾脏细胞凋亡
- Author:
Jian-Qing SHE
1
;
Bo-Wen LOU
;
Yue WU
;
Zu-Yi YUAN
Author Information
1. 西安交通大学第一附属医院心血管内科
- Keywords:
Erythropoietin;
Zebrafish;
Embryonic development;
Kidney;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2018;34(6):1067-1074
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To analyze zebrafish embryos and to identify erythropoietin (Epo) as an active renal anti-apoptotic factor in an Epo receptor (EpoR)-dependent manner. METHODS:For transient knockdown of Epo and EpoR in renal Tg (wt1b:EGFP) zebrafish reporter lines, the morpholino antisense oligonucleotide technique was used. Morphant zebrafish embryos were phenotypically analyzed using fluorescence microscopy. Apoptosis was determined by TUNEL assay and Annexin V staining. Western blot was used to identify Akt phosphorylation in Epo and EpoR knockdown zebrafish. RE-SULTS:Epo and EpoR zebrafish morphants exhibited pathophysiological changes within the pronephros, adversely affecting pronephric structure. Zebrafish embryos upon silencing of Epoa and EpoR showed a significant increase in the apoptosis within the zebrafish pronephros, consequently leading to renal pathophysiological effects. Decreased p-Akt was identified in Epo and EpoR knockdown zebrafish embryos. CONCLUSION:Epo is an essential regulator of renal development and function by interacting with its receptor EpoR and thereby repressing apoptosis, mechanistically by promoting Akt phospho-rylation.
