Effects of USP9X down-regulation on apoptosis and invasion ability of gastric carcinoma AGS cells
10.3969/j.issn.1000-4718.2018.06.013
- VernacularTitle:下调USP9X表达对胃癌AGS细胞凋亡和侵袭能力的影响
- Author:
Cai-Feng ZHANG
1
;
Yu HAN
;
Yong-Hua XIA
;
Xue-Fang DU
;
Huai-Cong XIAO
;
Run-Gen ZHAO
;
Li-Li ZHANG
;
Shuang-Mei YANG
Author Information
1. 新乡医学院第一附属医院消化内科
- Keywords:
Ubiquitin-specific peptidase 9,X-linked;
Gastric carcinoma;
Apoptosis;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2018;34(6):1037-1041
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of ubiquitin-specific peptidase 9, X-linked (USP9X) down-regula-tion on apoptosis and invasion ability in gastric carcinoma cells, and to explore its possible molecular mechanisms. METH-ODS:USP9X small interfering RNA (siRNA) and control siRNA were used to be transfected into gastric carcinoma AGS cells. The cells were divided into 3 groups, including untreated AGS group, control siRNA group and USP9X siRNA group. The expression of USP9X at mRNA and protein levels in the AGS cells with different treatments was determined by real-time PCR and Western blot. The cell viability was analyzed by CCK-8 assay. Flow cytometry and Boyden chamber were employed to examine the apoptosis and invasion ability of the AGS cells. RESULTS:USP9X siRNA significantly down-regulated the expression of USP9X at mRNA and protein levels in the AGS cells. Down-regulation of USP9X markedly induced apoptosis and reduced invasion ability of the gastric carcinoma AGS cells. Notably, down-regulation of USP9X sig-nificantly reduced the protein expression of Mcl-1 and MMP-2, but markedly increased the protein level of Bax. CON-CLUSION:USP9X may be a key regulator for apoptosis and invasion in gastric carcinoma.
