Calreticulin promotes migration and invasion of nasopharyngeal carcino-ma cells by inducing cell EMT
10.3969/j.issn.1000-4718.2018.05.024
- VernacularTitle:Calreticulin 通过诱导细胞EMT促进鼻咽癌迁移和侵袭
- Author:
Jun-Hui ZHU
1
;
Ming JIN
;
Hao QIU
;
He-Liang ZHANG
;
Wei MA
;
Xuan XIAO
;
-song Chen LI
;
Zhao-Yang LUO
;
Zhi-Wei ZHANG
Author Information
1. 南华大学医学院肿瘤研究所
- Keywords:
Nasopharyngeal carcinoma;
Calreticulin;
Epithelial-mesencymal transition;
Cell migration;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2018;34(5):925-929
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expression of calreticulin(CRT)in nasopharyngeal carcinoma tissues,ana-lyze the significance of clinical pathology and the influence on epithelial -mesencymal transition(EMT)of CNE2 cells. METHODS:The expression of calreticulin was detected by immunohistochemistry in 52 nasopharyngeal carcinoma and 57 nasopharyngeal benign tissues,and the significance of clinical pathology was evaluated.The calreticulin gene-specific small interfering RNA was constructed,and then was transfected into the NPC cell line CNE 2 using the cationic liposome meth-od.The effect of CRT on the morphological changes of the CNE 2 cells was observed under light microscope.The effect of CRT on the cell migration and invasion abilities of the CNE 2 cells was detected by Transwell migration and invasion assays. The expression of EMT-related proteins E-cadherin,vimentin,transforming growth factor(TGF)-βand matrix metallopro-teinase(MMP)-9 in the CNE2 cells was determined by Western blot.RESULTS:The positive expression rate of CRT in the benign lesion tissues was 19.29%(11/57),which was significantly increased in the nasopharyngeal carcinoma tissues as 82.69%(43/52).The expression rate of CRT was positively correlated with the stage of nasopharyngeal carcinoma and lymph node metastasis(P<0.05).Knockdown of CRT expression made the CNE 2 cells showing a spindle shape to a flat, cobblestone-like epithelial state change,arranged more compact,and the migration and invasion abilities were significantly decreased(P<0.05).Knockdown of CRT expression resulted in significant increase in the protein expression of E -cadhe-rin,and the decreases in the protein expression of vimentin, TGF-βand MMP-9 in the CNE2 cells(P<0.05).CON-CLUSION:Calreticulin expression in nasopharyngeal carcinoma is significantly higher and positively correlated with naso -pharyngeal carcinoma stage and lymph node metastasis.Calreticulin promotes cell migration and invasion of nasopharyngeal carcinoma CNE2 cells by inducing EMT.
