Effect of sinapine on H2O2-induced adipogenic differentiation of bone marrow mesenchymal stem cells
10.3969/j.issn.1000-4718.2018.05.023
- VernacularTitle:芥子碱对H2O2诱导BMSCs成脂分化的影响
- Author:
Wei-Ping HUANG
1
;
Ke LI
;
Ai-Jun LIU
;
Dong-Feng CHEN
;
Hong-Qi WANG
Author Information
1. 广州中医药大学基础医学院
- Keywords:
Sinapine;
Bone marrow mesenchymal stem cells;
Adipogenic differentiation;
Hydrogen peroxide;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2018;34(5):918-924
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of sinapine,an effective monomer of Chinese medicine,on hydro-gen peroxide(H2O2)-induced adipogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).METHODS:The undifferentiated rat BMSCs were identified and screened by flow cytometry.The adipogenic differentiation of BMSCs was induced by H2O2,and the toxicity of sinapine on BMSCs was tested by CCK-8 assay.After the modeling method and the concentration range of sinapine were determined,the lipid droplets in the cells were detected by Oil Red O semi-quanti-tative assay,and the optimal drug concentration was selected.Finally,Oil Red O assay was observed 24 h after drug inter-vention,and the expression of adipogenic differentiation-related proteins,adipocyte protein 2(aP2),peroxisome prolifera-tor-activated receptor γ(PPARγ)and glucose transporter 4(Glut4),at mRNA and protein levels in the BMSCs was deter-mined by qPCR and Western blot.RESULTS:Treatment with H2O2at 200 μmol/L for 1 h induced BMSCs to differentiate into adipocytes.Below the concentration of 40 μmol/L,sinapine had no toxicity to BMSCs.The best inhibitory concentra-tion of sinapine on adipogenic differentiation was at 15 μmol/L.The number of lipid droplets in sinapine(15 μmol/L) group was significantly lower than that in model group.In sinapine group,the expression of aP2,PPARγand Glut4 at mR-NA and protein levels was lower than that in model group(P<0.01).CONCLUSION: Sinapine inhibits H2O2-induced adipogenic differentiation of rat BMSCs.The mechanism may be related to the PPARγ/AMPK signaling pathway.
