Selective inhibition of endothelin receptor A improves white matter le-sions in hypertensive rats
10.3969/j.issn.1000-4718.2018.05.016
- VernacularTitle:选择性内皮素受体A拮抗剂改善高血压大鼠脑白质病变
- Author:
Di-Long WANG
1
;
Bao-Shan QIU
;
Jing LIN
;
Ying CAI
;
Yu-Hua FAN
Author Information
1. 中山大学附属第一医院神经内科
- Keywords:
White matter lesion;
Hypertension;
Endothelin-1;
Cognition disorder;
Endothelin receptor A
- From:
Chinese Journal of Pathophysiology
2018;34(5):878-883
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe whether selective inhibition of endothelin receptor A(ETRA)improves white matter lesions(WMLs),and explore the mechanism.METHODS:Sprague-Dawley rats(n=33)were randomly divided into sham operation group(n=9),treatment group[stroke-prone renovascular hypertensive rats-modified 2 vessel occlu-sion(RHRSP-modified 2VO)+ambrisentan(n=12)]and placebo group[RHRSP-modified 2VO +vehicle(n =12)].Drug and vehicle administration was performed from 17th to 20th week and monitoring of systolic arterial pressure was performed weekly.Morris water maze test was conducted to evaluate the function of cognition.The protein levels of en-dothelin-1(ET-1)in the cortex,corpus callosum and caudate putamen were quantitatively analyzed respectively.The se-verity of WMLs and the relationship between ET-1 and vessels were observed by the method of histopathology.RESULTS:The difference of systolic arterial pressure between treatment group and placebo group was not significant.The animals in treatment group exhibited shorter escape latency(P<0.05),more times of crossing platform(P<0.05), lower level of ET-1 in corpus callosum and caudate putamen(P<0.05),respectively,improved WMLs severity(P<0.05)and lower binding level of ET-1 to vessels compared with the placebo group.CONCLUSION: Selective inhibition of endothelin receptor A improves the severity of WMLs and ameliorates the cognitive function.
