Effects of kaempferol-3-O-rutinoside on proliferation,migration and TG-FBR1 signaling pathway activation in vascular smooth muscle cells
10.3969/j.issn.1000-4718.2018.05.010
- VernacularTitle:山柰酚-3-O-芸香糖苷对血管平滑肌细胞增殖、迁移及TGFBR1信号通路活化的影响
- Author:
Wen-Tong ZHANG
1
;
Jun LI
;
Yu-Ting WU
;
Hui-Jie FAN
;
Ling-Peng XIE
;
Zhang-Bin TAN
;
Yi-Ming BI
;
Bin LIU
;
Ying-Chun ZHOU
Author Information
1. 南方医科大学中医药学院
- Keywords:
Kaempferol-3-O-rutinoside;
Vascular smooth muscle cells;
Cell proliferation;
Cell migration;
TGFBR1 signaling pathway
- From:
Chinese Journal of Pathophysiology
2018;34(5):832-838
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of kaempferol-3-O-rutinoside(KR)on the proliferation,migration of vascular smooth muscle cells(VSMC)and the activation of transforming growth factor βreceptor 1(TGFBR1)signaling pathway in the cells.METHODS: The viability of VSMC was detected by MTT assay.The proliferation of VSMC was measured by EdU staining.The migration ability of VSMC was examined by Transwell assay.The protein levels of the mi-gration-associated proteins matrix metalloproteinase 2(MMP2)and matrix metalloproteinase 9(MMP9)were detected by Western blot.Molecular docking study was conducted to explore the interaction between KR and TGFBR 1.The protein le-vels of the phosphorylated TGFBR1,Smad2 and Smad3 were determined by Western blot.RESULTS: KR inhibited the viability of VSMC in a dose-and time-dependent manner.KR reduced the ratio of EdU-positive cells in a dose-dependent manner.KR dose-dependently suppressed the migration ability of VSMC and decreased the protein levels of MMP 2 and MMP9(P<0.05).KR docked into TGFBR1 with the binding energy of -9.804 kcal/mol by forming hydrogen bonds with SER-280,ARG-215,ASP-290 and LYS-335 of TGBFR1.KR dose-dependently suppressed the activation of TGFBR 1 and its downstream proteins Smad2 and Smad3(P<0.05).CONCLUSION: KR inhibits the proliferation and migration of VSMC,possibly via blocking the TGFBR1 signaling pathway.
