Norepinephrine attenuates injury of vascular endothelial cells induced by LPS

VernacularTitle:去甲肾上腺素减轻脂多糖所致内皮细胞损伤
Author: Jing HU ¹ ; Yan-Qiong LONG ; Yang YUE ; Zuo-Liang LIU
Author Information

1. 中南大学湘雅三医院
Keywords: Norepinephrine; Lipopolysaccharides; Endothelial damage; Sepsis
From: Chinese Journal of Pathophysiology 2018;34(4):735-738
CountryChina
Language:Chinese
Abstract: AIM: To investigate the effects of norepinephrine(NE)on vascular endothelial cell damage in-duced by lipopolysaccharides(LPS).METHODS: Human umbilical vein endothelial cells(HUVEC-12)were cultured with LPS at 100 mg/L to establish the cell damage model.Real-time PCR and Western blot were used to determine the ex-pressions of VE-cadherin at mRNA and protein levels.The levels of TNF-α,IL-1β,IL-2 and IL-10 in culture supernatant were measured by ELISA.The reactive oxygen species(ROS)production in the endothelial cells was detected by ROS as-say kit.RESULTS: LPS decreased both mRNA and protein levels of VE-cadherin accompanied by increased levels of TNF-α,IL-1β,IL-2 and intracellular ROS,and decreased level of IL-10 in the endothelial cells.NE reversed the expres-sion of VE-cadherin at mRNA and protein levels under the condition of LPS treatment in a dose -dependent manner,and al-so alleviated the intracellular oxidative stress.CONCLUSION: NE reverses the endothelial damage induced by LPS, which may be related to the up-regulation of VE-cadherin level and the decreases in oxidative stress and inflamatory media-tors.