Effect of sulodexide on apoptosis of human dermal microvascular endo-thelial cells exposed to hypoxia
10.3969/j.issn.1000-4718.2018.04.013
- VernacularTitle:舒洛地特对低氧状态下人真皮微血管内皮细胞凋亡的影响
- Author:
Fu-Qiang GUI
1
;
Hong LUO
;
Hong LIU
;
Hua ZHU
;
Mao ZHANG
;
Yu ZHAO
Author Information
1. 重庆医科大学附属第一医院血管外科
- Keywords:
Sulodexide;
Human dermal microvascular endothelial cells;
Hypoxia;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2018;34(4):657-662
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of sulodexide(SDX)on the apoptosis of human dermal microvascu-lar endothelial cells(HDMECs)exposed to hypoxia and its underlying mechanism.METHODS:The HDMECs were cul-tured and divided into normoxia control group cultured under normoxic condition;hypoxia control group cultured in a humid incubator maintained at 37 ℃with 5% CO2and 1% O2for 24 h;treatment groups treated with SDX at 0.25,0.5 and 1 LSU/mL for 24 h under hypoxic condition.The cell viability was measured by CCK-8 assay.The apoptotic rate of the HD-MECs was analyzed by flow cytometry.The activity of caspase-3 in HDMECs was examined by caspase-3 activity assay kit. The expression of pro-apoptotic factor P53,caspase-3,Bax and anti-apoptotic factor Bcl-2 at mRNA and protein levels was determined by real-time PCR and Western blot.RESULTS:SDX increased the viability of HDMECs exposed to hypoxia, but also decreased the apoptosis.Furthermore, SDX down-regulated the expression of pro-apoptotic factor P53, Bax and caspase-3 at mRNA and protein levels as well as the activity of caspase-3,while the expression of anti-apoptotic factor Bcl-2 was up-regulated.CONCLUSION:SDX significantly increases the viability and decreases the apoptosis of HDMECs ex-posed to hypoxia.Inhibition of the mitochondrial apoptosis pathway may be involved in the underlying mechanism.
