Role of microRNA-126-5p in myocardial injury induced by doxorubicin
10.3969/j.issn.1000-4718.2018.04.010
- VernacularTitle:MicroRNA-126-5p在阿霉素所致损伤心肌中的表达及作用
- Author:
Yu-Ting TANG
1
;
Yan-Juan LIU
;
Zhong-Yi TONG
;
Yuan-Bin LI
;
Qing-Lan LV
;
SUN-Hui
;
Xuan-You LIU
;
Mei-Dong LIU
;
Bi-Mei JIANG
;
Xian-Zhong XIAO
Author Information
1. 中南大学湘雅医学院病理生理学系
- Keywords:
MicroRNA-126-5p;
Adriamycin;
Myocardial injury;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2018;34(4):637-642
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expression of microRNA-126-5p during myocardial injury and its role in myo-cardial cell injury induced by adriamycin(also called doxorubicin, DOX).METHODS: The BALB/c mouse model of DOX-induced acute and chronic myocardial injury was established via intraperitoneal injection of DOX.HE staining was applied to observe the morphological changes of myocardial tissues.Lactate dehydrogenase(LDH)in serum was detected and PowerLab system was used to detect the influence of DOX on the changes of ±dp/dtmax.The expression of microRNA-126-5p in injured myocardial tissues and the H 9c2 cells exposed to DOX was detected by real-time PCR.Gain-and loss-of-function experiments were conducted to detect the role of microRNA-126-5p in H9c2 cells treated with DOX on LDH release and caspase-3 activation.RESULTS:In acute and chronic DOX myocardial damage models in mice,HE staining showed disarranged myocardial fibers, dissolved myofibril and inflammatory cell infiltration.Higher serum LDH level and lower ±dp/dtmaxin DOX-treated mice than those in normal mice were found.Compared with the normal mice, the expression level of microRNA-126-5p was significant increased in the myocardium with DOX-induced injury.Similarly,the expression level of microRNA-126-5p was significant increased in the H9c2 cells treated with DOX.In addition, over-expression of microRNA-126-5p decreased cell viability and promoted apoptosis,while microRNA-126-5p ablation promoted the viability and inhibited the apoptosis of H9c2 cells.CONCLUSION:The microRNA-126-5p expression is up-regulated in myocar-dial injury induced by DOX,and microRNA-126-5p inhibits cell viability and promotes apoptosis induced by DOX.
