Effects of marrow stromal cell-mediated microenvironment on human lung adenocarcinoma A549 cells
10.3969/j.issn.1000-4718.2018.04.004
- VernacularTitle:骨髓基质细胞介导的微环境对人肺腺癌A549细胞的作用
- Author:
Wang LI
1
;
Si-Cheng CHEN
;
Yan-Ting SUN
;
Ju-Qiong LI
;
Ying ZHU
;
Meng-Hao ZHANG
;
Bin CHEN
;
Qiong SHI
Author Information
1. 重庆医科大学检验医学院临床诊断教育部重点实验室
- Keywords:
Marrow stromal cells;
Lung cancer;
Tumor microenvironment;
MAPK/ERK signaling pathway
- From:
Chinese Journal of Pathophysiology
2018;34(4):599-604
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of marrow stromal cell line HS-5 on human lung adenocarcinoma A549 cells in the tumor microenvironment.METHODS:The effects of HS-5 cell-conditioned medium(HS-5-CM)on the viability and migration ability of A549 cells were detected by MTT assay and wound-healing assay.After treatment with HS-5-CM,the expression of CX3C chemokine receptor 1(CX3CR1)at mRNA level in the A549 cells was examined by qPCR. The protein levels of p-ERK and ERK in the A549 cells treated with MAPK/ERK pathway inhibitor U0126 were observed by Western blot,the migration ability of the A549 cells was measured by wound-healing assay,and the protein expression of CX3CR1 was determined by Western blot.RESULTS: HS-5-CM promoted the viability and migration ability of the A549 cells(P<0.01).The expression of CX3CR1 at mRNA level in the A549 cells was increased after treatment with HS-5-CM.MAPK/ERK inhibitor U0126 inhibited the activation of MAPK/ERK signaling pathway(P<0.01), and re-duced the migration ability(P<0.01)and the expression of CX3CR1(P<0.05)in the A549 cells.CONCLUSION:HS-5-CM significantly promotes the A549 cell viability and migration ability.Activation of MAPK/ERK signaling pathway and the expression of CX3CR1 may play a important role in this process.
