Protective effect of MUC2 in mice with colitis and the expression of anti-CBir1 antibody in serum
10.3969/j.issn.1000-4718.2018.03.025
- VernacularTitle:MUC2在结肠炎小鼠中的保护作用和血清抗CBir1抗体的表达
- Author:
Xin HE
1
;
Mu-Han ZHANG
;
Yao LU
;
Jiang-Ming LOU
;
Mei-Hua ZHAO
;
Na MA
;
Bai-Sui FENG
Author Information
1. 郑州大学第二附属医院消化内科
- Keywords:
Mucin 2;
Colitis;
CBir1 flagellin;
Lipopolysaccharides;
Ketotifen
- From:
Chinese Journal of Pathophysiology
2018;34(3):533-538,560
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the protective effect of mucin 2(MUC2)on intestinal mucosa of colitis model mice,and to explore the correlation between the expression of anti-CBir1 flagellin antibody and MUC2.METHODS:The mice were randomly divided into normal control group,2,4,6-trinitrobenzenesulfonic acid(TNBS)group,lipopolysaccha-ride(LPS)+ovalalbumin(OVA)+TNBS group and ketotifen+TNBS group.The expression of MUC2 in colon tissue was determined by PAS staining and immunohistochemistry, and the anti-CBir1 antibody level in the serum of mice in each group was measured by ELISA.RESULTS:The scores of disease activity index and histological index in TNBS group were higher than those in normal control group(P<0.05).The scores in LPS +OVA+TNBS group were much higher than those in TNBS group(P<0.05).However, the values in ketotifen +TNBS group were lower than those in TNBS group (P<0.05).PAS staining showed a decrease in goblet cells in TNBS group.Compared with TNBS group,the colonic mu-cosa integrity in LPS+OVA+TNBS group was destroyed, and the number of goblet cells in ketotifen +TNBS group in-creased significantly.Immunohistochemical staining showed that the expression of MUC 2 in the intestinal tract of each mo-del group was basically consistent with the results of PAS staining.The serum anti-CBir1 antibody level in TNBS group was higher than that in normal control group(P<0.05), and that in LPS+OVA+TNBS group was significantly higher than that in TNBS group(P<0.05),whereas that in ketotifen +TNBS group was decreased slightly(P<0.05).CONCLU-SION:MUC2 plays a protective role in the pathogenesis of colitis in mice,and there is a negative correlation between the expression of MUC2 and the bacterial flagellin in the intestinal mucosa of mice with colitis.
