Irbesartan alleviates hepatic steatosis in db/db mice by inducing auto-phagy
10.3969/j.issn.1000-4718.2018.03.023
- VernacularTitle:厄贝沙坦通过诱导自噬减轻db/db小鼠肝脏脂肪变
- Author:
Juan ZHONG
1
;
Yao QING
;
Shu-Yue WU
;
Wang-Qiu GONG
;
Hai-Bo LONG
Author Information
1. 南宁市第一人民医院
- Keywords:
Irbesartan;
Autophagy;
PI3K/Akt/mTOR signaling pathway;
Hepatic steatosis
- From:
Chinese Journal of Pathophysiology
2018;34(3):521-527
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of irbesartan on the fatty liver of db/db mice and whether autophagy is involved in the process.METHODS:Male db/db mice(n=24)were randomly divided into model group and irbesar-tan group,and 12 db/m mice with similar age and weight were selected as normal control group.After 16 weeks of inter-vention respectively,the fatty liver-related parameters including body weight, liver index, blood lipid, liver function and pathological changes in the liver were observed.The protein levels of p-PI3K,p-Akt,and p-mTOR,as well as Atg-7,bec-lin-1 and LC3B in the liver tissues were detected by Western blot,and the autophagosomes in the liver were observed under electron microscope.RESULTS:Compared with the model group,the body weight,liver index,blood lipids,alanine and aspartate aminotransferase were decreased in irbesartan group(P<0.05).Moreover,the pathological changes in the liver were significantly ameliorated in irbesartan group than that of model group.Importantly, the protein levels of p-PI3K, p-Akt and p-mTOR were decreased with irbesartan administration,while the expression of Atg-7,beclin-1 and LC3B-Ⅱwas increased(P<0.05),which resulted in a distinct increase in autophagosomes.CONCLUSION:Irbesartan alleviates he-patic steatosis in db/db mice by inhibiting the PI3K/Akt/mTOR signaling pathway and upregulating the protein expression of Atg-7,beclin-1 and LC3B-Ⅱ,thereby inducing autophagy in hepatocytes.
