miR-451 inhibits inflammatory responses in glomerular mesangial cells by targeting Psmb8 in diabetic nephropathy mice
10.3969/j.issn.1000-4718.2018.03.019
- VernacularTitle:miR-451通过靶向Psmb8抑制糖尿病肾病小鼠肾小球系膜细胞炎症反应
- Author:
Wen-Hao JIANG
1
;
Yan SUN
;
Rui PENG
;
Hui-Min PENG
;
Zheng ZHANG
Author Information
1. 重庆医科大学基础医学院
- Keywords:
Diabetic nephropathy;
MicroRNA-451;
Inflammation;
Proteasome subunit βtype 8;
Glomerular mesangial cells
- From:
Chinese Journal of Pathophysiology
2018;34(3):494-499
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of microRNA(miR)-451 by targeting proteasome subunit βtype 8 (Psmb8)on the inflammatory responses in mouse glomerular mesangial cells(MCs)under high-and low-glucose condi-tions.METHODS:The expression levels of miR-451,IL-18 mRNA and TNF-αmRNA were detected by qPCR.The pro-tein expression levels of IL-18,TNF-αand Psmb8 were determined by Western blot when miR-451 was over-expressed and down-expressed in the MCs.Moreover,the expression of IL-18 and TNF-αwas detected when Psmb8 was silenced by si-Psmb8 in MCs.RESULTS:The expression of miR-451 was significantly decreased in the MCs treated with high glucose compared with low glucose group(P<0.01).However,the expression of Psmb8 was increased in high glucose group as compared with low glucose group(P<0.01).Moreover, the expression levels of Psmb8, IL-18 and TNF-αwere signifi-cantly decreased when miR-451 was over-expressed in high glucose group(P<0.01).Additionally,the expression levels of IL-18 and TNF-αwere significantly reduced when Psmb8 was silenced in the MCs under high glucose condition.CON-CLUSION:miR-451 reduces the expression of inflammatory factors via targeting Psmb8 in the MCs under high glucose condition.Therefore,miR-451 may play a role in inflammation of diabetic nephropathy.
