Regulatory effects of miR-195 on biological behaviors of lung cancer A549 cells
10.3969/j.issn.1000-4718.2018.03.013
- VernacularTitle:miR-195对肺癌A549细胞生物学行为的调控作用
- Author:
Yi WANG
1
;
Rong ZHAN
;
Jian-Sheng GAO
;
Dong-Ying ZHANG
;
Hua LIU
Author Information
1. 广东药科大学附属第一医院干保科
- Keywords:
MicroRNA-195;
Lung cancer;
Cell proliferation;
Apoptosis;
Cell migration;
MYB gene
- From:
Chinese Journal of Pathophysiology
2018;34(3):458-463
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the regulatory effects of microRNA(miR)-195 on the biological behaviors, such as viability,apoptosis and migration, of lung cancer A549 cells, and to explore the related mechanisms.METH-ODS:After miR-195 mimics were transfected into the A549 cells,the cell viability, cell cycle distribution and apoptosis were measured by CCK-8 assay and flow cytometry.Transwell assay was used to detect cell migration ability.Furthermore, the protein levels of cyclin D1,CDK2,Bcl-2 and p-Rb/Rb were determined by Western blot.Dual-luciferase reporter as-say was used to screen and identify the possible target genes of miR-195.RESULTS: Over-expression of miR-195 in the A549 cells inhibited the cell viability and induced cell cycle arrest,accompanied with the decrease in the cell migration a-bility and the increase in the apoptotic rate(P<0.05).Furthermore,the protein levels of cyclin D1,CDK2,Bcl-2 and p-Rb were significantly decreased(P<0.05).Dual-luciferase reporter assay demonstrated that MYB was a potential target gene of miR-195.Over-expression of MYB in the A549 cells partially reversed the effects of miR-195 on the cell viability, apoptosis and migration.CONCLUSION: miR-195 inhibits lung cancer A549 cell growth and migration, and promotes cell apoptosis by targeting MYB gene.