Upregulated FAAH expression in PVN contributes to sympathoexcitation in rat with chronic heart failure
10.3969/j.issn.1000-4718.2018.03.001
- VernacularTitle:室旁核内脂肪酸酰胺水解酶上调介导慢性心衰大鼠交感神经兴奋亢进
- Author:
Ren-Jun WANG
1
;
Qin ZHOU
;
Xiao-Wei WEI
;
Hua LI
;
Yong-Bin ZHAO
;
Yun-Feng QI
;
Jian LUAN
;
Xiao-Fu ZHOU
Author Information
1. 吉林师范大学生命科学学院生物技术系
- Keywords:
Paraventricular nucleus;
Chronic heart failure;
Sympathoexcitation;
Fatty-acid amide hydrolase;
N-arachidonoylethandamide
- From:
Chinese Journal of Pathophysiology
2018;34(3):385-392
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression of fatty-acid amide hydrolase(FAAH)in paraventricular nu-cleus(PVN)and its contribution to renal sympathetic nerve activity in rats with chronic heart failure(CHF).METH-ODS:A rat model of CHF was established by ligation of the left coronary artery to induce acute myocardial infarction. Eight weeks after ischemia,the rat model of CHF was identified by echocardiogram and histopathological observation.The plasma level of norepinephrine(NE)was detected by ELISA.The protein expression levels of FAAH in the PVN were de-termined by Western blot.The N-arachidonoylethanolamide(AEA)generation in PVN was analyzed by high-performance liquid chromatography.After microinjection of AEA,PF3845(an FAAH inhibitor)or rAAV2-FAAH shRNA virus in PVN, the sympathetic drive indexes were recorded in different experiment groups.RESULTS: Compared with the rats in sham group,the cardiac function and AEA concentration in PVN were significantly reduced, while the plasma NE level and FAAH expression in PVN were obviously increased in the CHF rats(P<0.05).After microinjecion of PF3845, AEA or rAAV2-FAAH shRNA virus in PVN, the sympathetic drive indexes were decreased significantly and the cardiac function were improved in the CHF rats.CONCLUSION:Upregulated FAAH expression in PVN may result in sympathoexcitation in the rat with CHF.
