Effects of ClC-3 over-expression on structure and function of thyroid in mice
10.3969/j.issn.1000-4718.2018.02.023
- VernacularTitle:ClC-3氯通道过表达对小鼠甲状腺结构及功能的影响
- Author:
Qiu-Chan TAN
1
;
Zhan-Ru CHEN
;
Mei-Sheng YU
;
Xie-Chou LIANG
;
Chan ZHAO
;
Hong GAO
;
Yan-Fang ZHENG
;
Jia-Bao WU
;
Lin-Yan ZHU
;
Li-Wei WANG
;
Li-Xin CHEN
Author Information
1. 暨南大学基础医学院生理学系
- Keywords:
ClC-3;
Thyroid gland;
Transgenic mice
- From:
Chinese Journal of Pathophysiology
2018;34(2):335-339
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of ClC-3 gene over-expression on thyroid structure and function in mice. METHODS:Three-months-old FVB mice were used to study the difference of thyroid structure and function between wild-type(WT)mouse and ClC-3 transgene mice.The expression and distribution of ClC-3 in the thyroid of mice were deter-mined by the methods of qPCR,Western blot and immunofluorescence.Behavioral monitoring was performed on the daily activities of mice.Serum concentrations of total triiodothyronine(TT3), total thyroxine(TT4)and thyrotropin(TSH) were measured by ELISA.RESULTS:Compared with the WT group,the expression of ClC-3 in the thyroid of ClC-3 trans-gene group was significantly increased(P<0.05).The thyroid gland showed obvious hyperplasia and the folliculi glandu-lae thyreoideae was significantly bigger in ClC-3 transgene mice(P<0.05).The weight loss was increased in ClC-3 trans-gene mice(P<0.05).The expression of TT3 and TT4 were significantly higher than that of WT group(P<0.05),but the change of TSH was not obvious.CONCLUSION:ClC-3 over-expression results in thyroid hyperplasia and thyroid hor-mone secretion.This study suggests that ClC-3 is likely to be involved in the synthesis of thyroid hormones.
