Protective effect of procyanidins on PC12 cells exposed to Aβ25-35
10.3969/j.issn.1000-4718.2018.02.017
- VernacularTitle:原花青素对Aβ25-35作用下PC12细胞的保护作用
- Author:
Qin WANG
1
;
Yan YANG
;
Dong WANG
;
Jun AN
;
Li ZHANG
;
Mao-Bin YE
Author Information
1. 华中科技大学同济医学院附属武汉中心医院神经内科
- Keywords:
Procyanidins;
Alzheimer disease;
Reactive oxygen species;
Mitochondrial membrane potential;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2018;34(2):294-299
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the protective effect of procyanidins on the PC 12 cells exposed to Aβ25-35and the mechanisms.METHODS:Aβ25-35at 25 μmol/L was used to treat the PC12 cells for 48 h, and the PC12 cells were pretreated with procyanidins at 25,50 and 100 mg/L for 24 h.The cell vitality was measured by MTT assay.The content of reactive oxygen species(ROS)was detected by DCFH-DA staining.The change of mitochondrial membrane potential was examined by JC-10 staining.The apoptosis was analyzed by flow cytometry with Annexin V /PI double staining.The protein levels of activated caspase-3 was determined by Western blot.RESULTS:Under the exposure of the PC12 cells to Aβ25-35,procyanidins increased the cell viability,reduced intracellular ROS level, prevented mitochondrial membrane po-tential decline,attenuated the caspase-3 activation and inhibited the apoptosis of PC 12 cells(P<0.05 or P<0.01). CONCLUSION:Procyanidins have a significant protective effect on the PC 12 cells exposed to Aβ25-35.Its mechanism may be related to removing intracellular ROS induced by A β25-35, relieving the damage to the mitochondrial membrane, and thereby inhibiting cell apoptosis.
