Effects of curcumin on expression of caspase-3 , caspase-8 and caspase-9 in PC12 cells induced by Aβ25-35
10.3969/j.issn.1000-4718.2018.01.029
- VernacularTitle:姜黄素对Aβ25-35诱导的PC12细胞caspase-3、caspase-8和caspase-9表达的影响
- Author:
Hua SHANG
1
;
Xian-Hui REN
;
Hong-Xin YANG
;
Feng XU
Author Information
1. 牡丹江医学院第二附属医院 老年病科
- Keywords:
Curcumin;
Alzheimer disease;
Apoptosis;
β-amyloid protein 25-35;
Caspases
- From:
Chinese Journal of Pathophysiology
2018;34(1):168-172,182
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of curcumin on the viability ,the lactate dehydrogenase(LDH)re-lease,the apoptosis,and the activity and the expression levels of caspase-3,caspase-8 and caspase-9 of rat adrenal pheo-chromocytoma PC12 cells induced by β-amyloid protein 25-35(Aβ25-35 ).METHODS:The PC12 cells were treated with Aβ25-35.The viability and LDH release rates were measured by MTT assay and LDH kit ,respectively.The cells were ran-domly divided into blank control group ,model group,curcumin 10μmol/L group and curcumin 20μmol/L group.The ap-optotic rates were evaluated by flow cytometry with Annexin V-FITC/PI staining.The activities and expression levels of caspase-3,caspase-8 and caspase-9 were detected by colorimetric method and Western blot analysis.RESULTS:Com-pared with model group ,curcumin significantly increased the viability ,and decreased the LDH release rates and the apop-totic rates of the PC12 cells treated with Aβ25-35(P<0.01).Compared with model group,curcumin significantly decreased the activity and expression levels of caspase-3,caspase-8 and caspase-9(P<0.05 or P<0.01).CONCLUSION:Cur-cumin inhibits Aβ25-35-induced apoptosis of PC12 cells by inhibiting the expression of caspase-3,caspase-8 and caspase-9.
