Analyzing clinical characteristic of miR-196b in human colorectal cancer using TCGA and effect of miR-196 b on 5-FU resistance of CRC cell line
10.3969/j.issn.1000-4718.2018.01.026
- VernacularTitle:利用TCGA数据库分析miR-196b在结直肠癌患者中的临床表达特征并探索miR-196b的体外抗5-FU作用
- Author:
Jia CHE
1
;
Lan-Lan HUANG
;
Qiong LOU
;
Xiang-Ling YANG
;
Huan-Liang LIU
Author Information
1. 中山大学附属第六医院 广东省胃肠病学研究所
- Keywords:
Colorectal cancer;
The Cancer Genome Atlas;
MicroRNA-196b;
5-fluorouracil;
Drug resistance
- From:
Chinese Journal of Pathophysiology
2018;34(1):152-157
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the clinical characteristics of microRNA(miR)-196b in colorectal cancer(CRC)and to study its biological function in 5-fluorouracil(5-FU)resistance.METHODS:miRNA sequence dataset and the corresponding clinical data of CRC patients were downloaded from The Cancer Genome Atlas(TCGA).Expression level and clinical characteristics of miR-196b in CRC patients were analyzed using SPSS 17.0.CRC cell line overexpres-sing miR-196b was established using transient transfection method.MTS test was used to evaluate the effect of miR-196b overexpression on 5-FU resistance.RESULTS:miR-196b expression was associated with lymph node metastasis and TNM stage(P<0.05),but not related with age and sex.Lymph node metastasis and distant metastasis were independent prog-nostic factors for rectal patients(P<0.05).The expression level of miR-196b was not associated with survival condition of rectal patients.The viability of the cells overexpressing miR-196b treated with different concentrations of 5-FU was signifi-cantly higher than that in control group(P<0.05).CONCLUSION:miR-196b may be a potential biomarker of TNM stage and lymph node metastasis in CRC.miR-196b increases the 5-FU resistance of CRC cells.
