Protective effect of DIZE on heart function of rats with diabetic cardio-myopathy
10.3969/j.issn.1000-4718.2018.01.025
- VernacularTitle:DIZE对糖尿病心肌病大鼠心功能的保护作用
- Author:
Min YANG
1
;
Xin-Ran CAO
;
Yuan-Yuan WANG
;
Xiao-Qiong WANG
;
Shi-Ran YU
;
Bo DONG
;
Jing GAO
Author Information
1. 山东中医药大学
- Keywords:
Diabetic cardiomyopathy;
Angiotensin-converting enzyme;
Diminazene aceturate;
Inflammation;
Heart function
- From:
Chinese Journal of Pathophysiology
2018;34(1):147-151,177
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observed the protective effect of diminazene aceturate(DIZE),an angiotensin-converting enzyme 2(ACE2)activator,on rats with diabetic cardiomyopathy(DCM).METHODS:Male Wistar rats(n=30)were randomly divided into normal control group ,DCM group and DIZE treatment group(DIZE group).The rats in DCM group and DIZE group were intraperitoneally injected with streptozotocin(65 mg/kg )to establish diabetic model.After 12 weeks,the diabetic rats were infused with DIZE at 15 mg· kg-1 · d-1 or the same volume of saline for 4 weeks using os-motic minipump.The cardiac function was measured at the end of the 16th week.The methods of Mason staining and HE staining were used to observe the morphological changes of the myocardial tissue.Western blot ,ELISA and immunohisto-chemistry were used to observe the changes of ACE2,angiotensin(Ang)Ⅱ,Ang-(1-7),interleukin(IL)-1,IL-6 and connective tissue growth factor(CTGF).RESULTS:DIZE significantly improved the expression of ACE 2 in diabetic rats(P<0.05).Compared with DCM group,the levels of IL-1 and IL-6 in DIZE group were significantly decreased ,and the cardiac function in DIZE group was significantly improved(P<0.05).CONCLUSION:ACE2 endogenous agonist DIZE significantly increases the ACE 2 level and reduces the level of inflammation ,thus protecting the heart function of DCM rats.
