Effect of acteoside on behavioral changes and endoplasmic reticulum stress in prefrontal cortex of depressive rats
10.3969/j.issn.1000-4718.2018.01.017
- VernacularTitle:毛蕊花糖苷对抑郁症大鼠行为学和前额叶皮层内质网应激的影响
- Author:
Hai-Feng DENG
1
;
Man-Li SUN
;
Hao CHEN
;
Xing-Hong WANG
;
Qiong WU
;
Quan-Zhong CHANG
Author Information
1. 漯河医学高等专科学校
- Keywords:
Depression;
Acteoside;
Endoplasmic reticulum stress;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2018;34(1):101-106
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of acteoside on behavioral changes and endoplasmic reticulum stress(ERS)in prefrontal cortex of depressive rats.METHODS:Sprague-Dawley(SD)rats(n=108)were randomly divided into 6 groups:control group,model group,fluoxetine(20 mg/kg)group,low-dose(30 mg/kg)acteoside group,medium-dose(60 mg/kg)acteoside group and high-dose(120 mg/kg)acteoside group,with 18 rats in each group.The depres-sive-like rat model was established by chronic unpredictable mild stress(CUMS)combined with solitary way for 28 d.The rats in fluoxetine group and acteoside groups were treated with fluoxetine(20 mg/kg)or acteoside(30 mg/kg,60 mg/kg and 120 mg/kg)once daily by intragastric administration for 3 weeks.The rats in control group and model group were both given equal volume of saline by intragastric administration for 3 weeks.The behavioral changes were detected by the open-field test and sugar preference experiment.The protein expression of glucose-regulated protein 78(GRP78 )and C/EBP homologous protein(CHOP)was assessed by immunofluorescence and Western blot.The caspase-3 activity was measured by spectrophotometer.RESULTS:Compared with control group ,the total distance ,time spent in the center and sugar in-take were all decreased ,the expression of GRP78 and CHOP was increased ,and the activity of caspase-3 was increased in model group ,fluoxetine group and acteoside groups(P<0.05 ).Compared with model group ,the total distance ,time spent in the center and sugar intake were increased ,the expression of GRP78 and CHOP was reduced ,and the activity of caspase-3 was decreased(P<0.05)in fluoxetine group and acteoside groups.CONCLUSION:Acteoside improves de-pressive-like behaviors in depressive rats ,which may be related to the inhibition of ERS and neuronal apoptosis in prefron-tal cortex.
