Imperatorin enhances anti-tumor effect of TRAIL on breast cancer by upregulating expression of DR5 on cell surface
10.3969/j.issn.1000-4718.2018.01.014
- VernacularTitle:欧前胡素通过上调细胞表面死亡受体5的数量增强TRAIL对乳腺癌细胞的杀伤活性
- Author:
Zheng-Yang XU
1
;
Rui-Ping REN
;
Peng WAN
;
Zu-Guo YUAN
Author Information
1. 宁波市鄞州人民医院放化疗科
- Keywords:
Imperatorin;
Death receptor 5;
TNF-related apoptosis-inducing ligand;
Synergistic effect;
Breast cancer
- From:
Chinese Journal of Pathophysiology
2018;34(1):81-86
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the synergistic effect of imperatorin on enhancing the anti -tumor effect of TNF-related apoptosis-inducing ligand(TRAIL)on breast cancer and the mechanisms.METHODS:T-47D and MCF-7 breast cancer cells were divided into control group ,imperatorin group,TRAIL group,imperatorin+TRAIL group and imperatorin+TRAIL+death receptor 5(DR5)siRNA group.The viability of T-47D and MCF-7 cells was measured by MTT assay. The apoptosis and mitochondrial membrane potential in T-47D cells were analyzed by flow cytometry.Western blot and flow cytometry analysis were performed to evaluate the expression of DR 5 on T-47D cell surface and the activation of caspase-8 and caspase-3.RESULTS:Imperatorin significantly enhanced the inhibition of cell viability induced by TRAIL of T -47D and MCF-7 cells,and significantly increased the apoptosis of T-47D cells induced by TRAIL.Imperatorin treatment ob-viously induced upregulation of DR5 expression and production of reactive oxygen species in the T-47D cells.In addition,imperatorin enhanced the TRAIL-induced damage of mitochondrial membrane potential and activation of caspase -8 and caspase-3.CONCLUSION:Imperatorin enhances the anti-tumor effect of TRAIL on breast cancer via upregulating the ex-pression of DR5.
