Quality Evaluation of Shuwei Mixture Based on Multi-index Constituents
10.3870/j.issn.1004-0781.2018.11.025
- VernacularTitle:基于多指标成分的舒胃合剂质量评价
- Author:
Xiaojing NIU
1
;
Junhan SHI
;
Xiaoying DUAN
Author Information
1. 河南中医药大学第一附属医院药学部
- Keywords:
Shuwei mixture;
Sinapine cyanide sulfonate;
Magnolol;
Honokiol;
Hesperidin;
Naringin;
Neohesperidin;
Quality evaluation
- From:
Herald of Medicine
2018;37(11):1396-1400
- CountryChina
- Language:Chinese
-
Abstract:
Objective The quality evaluation of the Shuwei mixture was determined by the content of the six components of sinapine cyanide sulfonate, magnolol, honokiol, hesperidin, naringin and neohesperidin. Methods RP-HPLC method was used.The separation was performed on a Agilent ZORBAX Eclispse SB-C18column (4.6 mm×250 mm,5 mm),the mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid with gradient elution at the flow rate of 1.0 mL·min-1.The detection wavelength was 326 nm ( sinapine cyanide sulfonate ), 294 nm ( magnolol, honokiol ) and 283 nm ( naringin, neohesperidin).The column temperature was kept at 30 ℃. Results The sinapine cyanide sulfonate, magnolol, honokiol, hesperidin,naringin and neohesperidin all had good linear relationship in the ranges of 0.049 6-1.24,0.048 2-1.205,0.060 5-1.512 5,0.187 2-4.68,0.131 6-3.29,0.197-4.925 μg.The average recoveries were 100.66%, 99.86%, 101.37%, 102.41%, 99.01%, 102.05%, respectively, RSD were 0.82%, 1.89%, 2.56 %, 0.74%, 1.54%, 0.99%, respectively. Conclusion The method is simple,accurate,reproducible and nice to the separation,and can be used for the quality evaluation of Shuwei mixture.
