Determination of Isochlorogenic Acid A and Isochlorogenic Acid C in Vernonia Anthelmintica by High Performance Liquid Chromatography

VernacularTitle:高效液相色谱法测定驱虫斑鸠菊中异绿原酸A和异绿原酸C含量
Author: Jiayuan CHEN ¹ ; Shixia HUO ; Ming YAN ; Zhiyuan SONG ; SILAFU·Aibai
Author Information

1. 石河子大学药学院
Keywords: Vernonia anthelmintica; Isochlorogenic acid A; Isochlorogenic acid C; Chromatograph,high performance liquid
From: Herald of Medicine 2018;37(11):1393-1396
CountryChina
Language:Chinese
Abstract: Objective To establish the chromatographic conditions of isochlorogenic acid A and isochlorogenic acid C in vernonia anthelmintica. Methods By changing the mobile phase,flow rate,column temperature and other chromatographic conditions,the best chromatographic conditions was we pursued to established. Results The linear relationship between the concentration of isochlorogenic acid A and the peak area was between 5. 825-69. 9 μg·mL-1, and the concentration of isochlorogenic acid C,was between 5.15-61.80 μg·mL-1and the peak area was good . The sample recovery rates of the two groups were 98.70%-101.92%(RSD＝1.04%,n＝9)、95.99%-102.52%(RSD＝1.90%,n＝9). Conclusion The method is simple,rapid, accurate and reliable for the determination of isochlorogenic acid A and isochlorogenic acid C in Vernonia anthelmintica and also for the quality control of the raw material.