Simultaneous Determination of Five Components in Fufang Heishen Oral Liquid by Dual-wavelength HPLC
10.3870/j.issn.1004-0781.2018.11.023
- VernacularTitle:双波长高效液相色谱法同时测定复方黑参口服溶液中5种成分含量
- Author:
Jia YAN
1
;
Guixiang ZHONG
;
Qiaoting TAN
;
Hongtao SONG
;
Xin ZHOU
Author Information
1. 南京军区福州总医院药学科
- Keywords:
Fufang Heishen oral liquid;
Content determination;
Chromatography,high performance liquid
- From:
Herald of Medicine
2018;37(11):1389-1392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method that could detect 5 components of Fufang Heishen oral liquid simultaneously. Methods The component was performed by high performance liquid chromatography ( HPLC) equipped with Agilent Hypersil ODS (4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile-0.1% Phosphoric acid with gradient elution.The flow rate was 1.0 mL·min-1with the 210 nm and 270 nm detection wavelength,20 μL injection volume and 30 ℃column temperature. Results A good linear relationship was observed with the range of 7.12-85.44 mg·L-1for Harpagide (r＝0.999 9),2.50-30.00 mg·L-1for Harpagoside(r＝0.999 8),25.35-304.20 mg·L-1for Cinnamic acid(r＝0.999 7),0.73-8.70 mg·L-1for Tectoridin(r＝0.999 7)and 1.20-14.40 mg·L-1for Irisflorentin(r＝0.999 8).The average recovery of each detected component of Fufang Heishen Oral Liquid was 98.8%,102.7%,98.8%,99.3%,99.9% the RSD were 1.23%,2.89%, 2.60%,1.44%,2.84%(n＝6). Conclusion The method is simple,rapid and accurate and can be used to detect the content of Harpagide,Harpagoside,Cinnamic acid,Tectoridin and Irisflorentin of Fufang Heishen Oral Liquid.
