Determination of Nigeglanoside in Seeds of Nigella glandulifera by HPLC
10.3870/j.issn.1004-0781.2018.11.019
- VernacularTitle:高效液相色谱法测定黑种草子中黑种草苷含量
- Author:
Yujiao ZHAN
1
;
Chenyang LI
;
Xu HU
;
Yan CHEN
;
Jun ZHAO
;
Fang XU
Author Information
1. 新疆维吾尔自治区药物研究所
- Keywords:
Seeds of Nigella glandulifera;
Nigeglanoside;
Content determination;
Chromatography,high performance liquid
- From:
Herald of Medicine
2018;37(11):1374-1376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of nigeglanoside in seeds of Nigella glandulifera. Methods The content of nigeglanoside was determined by HPLC.The separation was performed on a C18column ( YMC-Pack ODS-A,250 mm×4.6 mm,5 μm) with a gradient elution system of acetonitrile and 0.017 5 mol·L-1acetic acid solution at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 290 nm,and column temperature was 30 ℃. Results The linear range of nigeglanoside was 0.01-0.30 mg·mL-1(R2＝0.9991).The RSDs of precision,stability and repeatability were all less than 2%.The average recovery was 96.66% (RSD＝1.25%,n＝6). Conclusion The method is accurate and reproducible. It is effective in controlling the quality of seeds of Nigella glandulifera .
