Simultaneous Determination of 19 Chemical Drugs in Traditional Chinese Medicines and Health Products for Treating Rhinitis by UPLC-MS/MS
10.3870/j.issn.1004-0781.2018.03.020
- VernacularTitle:超高效液相色谱-串联质谱法同时测定抗鼻炎中成药及保健品中19种化学药物
- Author:
Chengxian ZHANG
1
;
Xiaoping CHEN
;
Hongxia YANG
;
Chujian CHEN
;
Lu ZHANG
;
Weijin XU
Author Information
1. 浙江省湖州市食品药品检验研究院
- Keywords:
Anti-rhinitis Traditional Chinese Medicines;
Health products;
UPLC-MS/MS
- From:
Herald of Medicine
2018;37(3):356-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a UPLC-MS/MS method for simultaneous determination of 19 chemical drugs in Traditional Chinese Medicines and health products for treating rhinitis. Methods Separation was performed on Waters ACQU-ITY UPLC BEH-C18column(2.1 mm×100 mm,1.7 μm) with 0.1% formic acid acetonitrile solution and 0.1% formic acid aque-ous solution as the mobile phase by gradient elution.The flow rate was 0.3 mL·min-1.The column temperature was set at 35 ℃. The detection was performed by the positive ion electrospray ionization (ESI+) under multiple reactions monitoring (MRM) mode. Results The linear relationships of 19 chemical drugs were good in respective ranges with correlation coefficients higher than 0.995.The average recoveries of the low,medium,and high level were in the range of 84.9%-111.1%,and the RSDs were less than 5.2%.The limits of detection (LOD) and the limits of quantitation (LOQ) were in the range of 0.03-0.78 ng·mL-1 and 0.13-1.82 ng·mL-1,respectively. Conclusion The method is convenient,rapid,accurate,and sensitive,which can be used for the determination of chemical drugs added illegally in traditional Chinese medicines and health products for treating rhini-tis.
