Effects of Immunoglobulin G on TNF-α Secretion of Human Peripheral Blood Mono-nuclear Cells in Type 2 Diabetes Mellitus Complicated with Alzheimer's Disease
10.3870/j.issn.1004-0781.2018.03.005
- VernacularTitle:免疫球蛋白G对2型糖尿病合并阿尔茨海默病患者外周血单核细胞分泌TNF-α的影响
- Author:
Hongxia SHUAI
1
;
Juan DING
;
Ji ZHANG
Author Information
1. 湖北文理学院附属医院内分泌科
- Keywords:
Diabetes mellitus,type 2;
Alzheimer's disease;
Mononuclear cell;
Tumor necrosis factor-α
- From:
Herald of Medicine
2018;37(3):293-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of immunoglobulin G on tumor necrosis factor (TNF-α) secretion of human peripheral blood mononuclear cells(PBMCs) in type 2 diabetes mellitus complicated with Alzheimer's disease(AD). Methods A total of 20 cases were chosen in each group from patients with type 2 diabetes mellitus complicated with Alzheimer' s disease and patients with type 2 diabetes mellitusm.PBMCs were isolated using density gradient centrifugation method and incu-bated with phytohemagglutinin (PHA) as well as immunoglobulin G extracted from blood serum of the patients with type 2 diabe-tes mellitus.TNF-α concentration in supernatant was measured by enzyme linked immunosorbent assay (ELISA). Results TNF-α concentration in supernatant of PBMCs was significantly increased in type 2 diabetes mellitus complicated with AD com-pared with that in type 2 diabetes mellitus (P<0.01).IgG further increased TNF-α concentration in type 2 diabetes mellitus and type 2 diabetes mellitus complicated with AD(P<0.01). Conclusion IgG extracted from the serum of type 2 diabetes mellitus patients can promote TNF-α secretion in PBMCs of type 2 diabetes mellitus complicated with AD.Immunity injury could play a role in the development of type 2 diabetes mellitus complicated with AD by promoting inflammatory reaction.
