Purification Process of Polyphenols Group in Ilex paraguariensis Based on Fingerprint
10.3870/j.issn.1004-0781.2018.02.022
- VernacularTitle:基于指纹图谱的马黛茶中多酚类物质组纯化工艺
- Author:
Xiaojing CAI
1
;
Hua ZHANG
;
Haiyan SHI
;
Chengbo ZHANG
;
Nan XU
Author Information
1. 山东中医药大学药学院
- Keywords:
Ilex paraguariensis;
Total polyphenols;
Chlorogenic acid;
Macroporous resin;
Fingerprint
- From:
Herald of Medicine
2018;37(2):231-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the optimum technological conditions of macroporous resin separation and purification of polyphenol composition. Methods Fingerprint of Ilex paraguariensis was established by HPLC method. The best resin, loading conditions and elution requirements were screened with chlorogenic acid and elution rate of total active constituents served as the index. Results The optimum technological conditions were as follows:the separation carrier was D101 macroporous resin,the concentration of the sample liquid was 0.10 g?mL-1 ,the resin adsorption quantity of crude drugs was 1.5 g?g-1 ,the impurity was removed by four times of amount of water at first,and then eluted with four times the amount of 70％ ethanol in a gradient manner.After purification,the elution rate of chlorogenic acid was up to 82.83％,and the elution rate of total effective component was about 72. 48％. The resemblance of HPLC fingerprint was over 0. 98, and the components were balancedly recoveried. Conclusion The purification process of total polyphenols in Ilex paraguariensis by D101 macroporous resin was simple,reliable,and can be used for large-scale production.
