Evaluation of Measurement Uncertainty for the Determination of Flavonoids in Loquat Honey by UV Spectrophotometry
10.3870/j.issn.1004-0781.2018.02.020
- VernacularTitle:紫外-可见分光光度法测定 枇杷蜜中总黄酮含量不确定度评定
- Author:
Suqin HE
1
;
Xiaoxiao WANG
;
Yuchang XU
Author Information
1. 浙江省金华市中心医院药剂科
- Keywords:
Loquat honey;
Totle flavonoids;
Quercetin;
UV spectrophotometry;
Uncertainty
- From:
Herald of Medicine
2018;37(2):223-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the measurement uncertainty of flavonoids in loquat honey by UV spectrophotometry. Methods Through analyzing the whole process of the total flavonoids in loquat honey by UV spectrophotometry detection,the mathematical model was established,the uncertainty factors were determined,and each uncertainty was evaluated.Then the combined uncertainty was calculated and the expanded uncertainty of the measurement results at the 95％ confidence interval was given. Results The measurement uncertainty of the totle flavonoids content in loquat honey by UV spectrophotometry was (38.497±5. 674) μg?g-1 . Conclusion The uncertainty evaluation method is suitable for determination of the total flavonoids in loquat honey by UV method,and the uncertainty is mainly introduced by preparation of reference stock solution and standard curve fitting.
