Effects of CCR10 Expression on Proliferation, Migration and Invasion of NSCLC Cells
10.11969/j.issn.1673-548X.2018.05.023
- VernacularTitle:CCR10的表达对非小细胞肺癌细胞在增殖、迁移和侵袭的影响
- Author:
Zhiliang HOU
1
;
Wenbo WANG
Author Information
1. 450002,河南省胸科医院
- Keywords:
non-small cell lung cancer;
CCR10;
Proliferation;
Migration;
Invasion
- From:
Journal of Medical Research
2018;47(5):94-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of CCR10 expression on proliferation,migration and invasion of NSCLC cells.Methods CCR10 siRNA was conducted into humanlung adenocarcinoma cellA549 (A549) to interfere with the normal expression of CCR10,and then to study how dose CCR10 have effects on cell proliferation,migrationand invasion in NSCLC.Results The results of MTT showed that the proliferation rate of A549 conducted withCCR10 siRNA was significantly decreased compared with Negative Control (NC) and Mock(P ＜ 0.05).The proliferation rate of A549 in NC compared with Mock has nostatistical significance (P ＞ 0.05).The results of Transwell cell migration assay showed that the migration rate of A549 conducted with CCR10 siRNA was significantly decreased compared with NC and Mock(P ＜ 0.05).The proliferation rate of A549 in NC compared with Mock has nostatistical significance (P ＞0.05).The results of Transwell cell invasion assay showed that the invasion rate ofA549 conducted with CCR10 siRNA was significantly decreased compared with NC and Mock(P ＜ 0.05).The invasion rate of A549 in NC compared with Mock has nostatistical significance (P ＞0.05).Conclusion CCR10 may contribute to cell proliferation,migration and invasion in NSCLC,and associated with the occurrence and development of NSCLC.
