Effects and Its Mechanism of Different Dental Alloys on Proliferation and Apoptosis of Fibroblast
10.11969/j.issn.1673-548X.2018.01.025
- VernacularTitle:不同齿科合金对成纤维细胞增殖凋亡的影响及机制研究
- Author:
Shubao CHEN
1
;
Rui ZHANG
;
Rui LI
Author Information
1. 473000,南阳医学高等专科学校第二附属医院口腔科
- Keywords:
Dental alloys;
Fibroblasts;
Proliferation;
Apoptosis
- From:
Journal of Medical Research
2018;47(1):103-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and its mechanism of different dental alloys on proliferation and apoptosis of fibroblast.Methods Mouse fibroblast L929 cells were divided into negative control group,gold alloy group,Ni Cr alloy group and copper alloy group.Cell proliferation was detected by MTT assay.Cells were cultured for 48h.Cell cycle and apoptosis rate were detected by flow cytometry.The expression of Bcl-2,Bax,cleaved caspase-3 protein were detected by Western blot.Results Cell proliferation rate had no significant difference in gold alloy group and Ni Cr alloy group and negative control group (P ＞ 0.05),while was significantly lower in copper alloy group than the negative control group (P ＜ 0.01).G1 phase,S phase and G2 phase cells between gold alloy group and the negative control group had no significant difference (P ＞ 0.05),while G1 phase cells in Ni Cr alloy and copper alloy group was significantly lower than negative control group.S phase and G2 phase cells were significantly higher than the negative control group (P ＜ 0.05 or P ＜0.01).Cell apoptosis rate and the expression of Bcl-2,Bax,cleaved caspase-3 protein between gold alloy group and negative control group had no significant difference (P ＞ 0.05).Cell apoptosis rate and the expression of Bax,cleaved caspase-3 protein between Ni Cr alloy and copper alloy group were significantly higher than the negative control group.Bcl-2 protein expression was significantly lower than the negative control group (P ＜ 0.01).Conclusion The gold alloy,Ni Cr alloy and copper alloy can influence proliferation and apoptosis of fibroblast.The gold alloy group is minimum.Different dental alloys leads to the possible mechanism of fibroblast apoptosis was regulation of Bcl-2,Bax,cleaved caspase-3 protein expression.
