Osteoblast-derived IL-7 Prohibits Bone Formation through Activating mTORC1 Signaling
10.11969/j.issn.1673-548X.2017.12.039
- VernacularTitle:成骨细胞来源的IL-7通过激活mTORC1信号通路抑制骨形成
- Author:
Hualei WANG
1
Author Information
1. 473000,南阳市中心医院骨外科
- Keywords:
Osteoblast;
Interleukin-7;
mTOR;
Bone formation
- From:
Journal of Medical Research
2017;46(12):157-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation of bone formation by osteoblast-derived interleukin-7 (IL-7) trough the mammalian target of rapamycin (mTOR) signaling pathway.Methods The lentiviral vector with encoding frame of IL-7 gene was transfected into mouse MC3T3-E1 osteoblasts,and the expression levels of IL-7 mRNA and protein were detected,respectively,by qRT-PCR and ELISA method.Western blot method was used to detect the expression levels of P-S6,S6,collagen Ⅰ (Col Ⅰ) and Osteocalcin (Ocn) in MC3T3-E1 cells and in MC3T3-E1 cells stimulated by 0.1nmol/L mTORC1-specific inhibitor rapamycin.Results The IL-7 mRNA level of MC3T3-E1 cells transfected with upregulated 4.6 times,and ELISA assay showed an increase of 3.9 times of IL-7 level in osteoblastic supernatants.After transfected using lentiviral vector with encoding frame of IL-7 gene,the expression levels of P-S6 protein increased significantly,while the expression levels of Col Ⅰ and Ocn protein decreased significantly.However,rapamycin treatment of over-expressed-IL-7 MC3T3-E1 cells resulted in a decline of P-S6 protein levels,but an increase of Col Ⅰ and Ocn protein levels.Conclusion Osteoblast-derived IL-7 may inhibit its maturation by promoting mTORC1 signaling pathway.
