Brucein D Inhibits Proliferation and Induces Apoptosis in Colon Adenocarcinoma Cell Line HT29
10.11969/j.issn.1673-548X.2017.12.022
- VernacularTitle:鸦胆子素D对结肠癌HT29细胞株增殖的抑制及诱导其凋亡的机制
- Author:
Yanyan ZHENG
1
;
Jingjing CHEN
;
Zhaohong WANG
Author Information
1. 325000,温州市中西医结合医院肛肠外科、温州医科大学附属第二医院肝胆外科
- Keywords:
Brucein D;
Colon cancer;
Proliferation;
Apoptosis;
JNK;
p-JNK
- From:
Journal of Medical Research
2017;46(12):85-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate that brucein D (BD) possesses potent anti-colon cancer activity by inhibiting proliferation and inducing apoptosis in colon adenocarcinoma cell.Methods After human colon cancer cell line PANC-1 was treated with BD (0,5,10,20,30,80)μmol/L for 24,48,72h,the cell proliferation was detected by cell counting kit-8 (CCK-8)assay.The apoptosis of colon cancer cells was detected using Annexin V-FITC/PI.The protein expressions of JNK and p-JNK were detected using Western blot.Results After human colon cancer cell line PANC-1 was treated with BD for 24,48,72h,the cell survival rates decreased lower and apoptosis rate increased in concentration-and time-dependent manners.AS the concentration of BD got higher or the time got longer,the protein expression of JNK was down-regulated and that of p-JNK up-regulated significantly.In vivo,with the prolonged administration,the mean volume of xenograft tumor grew slowly.The protein expression of JNK was down-regulated and that of p-JNK up-regulated significantly in colon xenograft tumors cells.Conclusion With the increase of time and BD's concentration,the survival rates of colon cancer HT29 cells could be lower.Instead,the apoptosis rate has increased in a concentration-time-dependent manners.
