STC2 Suppresses Breast Cancer Cell Migration and Invasion Via the PKC-Claudin-1-mediated Signaling

VernacularTitle:斯钙素2对乳腺癌细胞侵袭、转移的影响及其机制研究
Author: Zhaoze GUO ¹ ; Guozhu XIE ; Ya LI ; Zhangsheng YE
Author Information

1. 南方医科大学南方医院
Keywords: Breast cancer; Stanniocalcin 2; Migration and invasion; EMT; PKC; Claudin-1
From: Journal of Medical Research 2017;46(12):63-67
CountryChina
Language:Chinese
Abstract: Objective To study the effect of STC2 on invasion and metastasis of breast cancer cells and its mechanism.Methods By 231 HM shRNA interference cell STC2,we observed fiber cells expressing form,number plate cloning experiment statistical analysis.Immune co-precipitation (CO-IP) for testing related protein expression.Results By 231 HM STC2 cells expressing shRNA interference,231 HM presents high transfer characteristics of fiber cell morphology,cell invasion and metastasis ability of increase at the same time;On the contrary,after 231 cells expressing STC2,cell invasion and metastasis ability induced.In addition,after reducing STC2 expression of 231 HM cells,its resistance to radiation-inducled apoptosis and expression of STC2 after 231 reduced,ability to resist radiation induced apoptosis of cells induled too.Conclusion Collectively,these results indicate that STC2 may inhibit EMT at least partially through the PKC/Claudin-1-mediated signaling in human breast cancer cells.