The expression and correlation of BMI1 gene in gastrointestinal stromal tumor
10.16571/j.cnki.1008-8199.2018.06.014
- VernacularTitle:BMI1基因在胃肠道间质瘤中的表达及其相关性
- Author:
Guo-Ding XU
1
;
Hong-Mian JIANG
;
Mao-Guang ZHU
;
Shi-Long ZHU
;
Ze SU
;
Chun-Ling LI
;
Hai HUANG
Author Information
1. 南宁市第一人民医院肝胆腺体外科
- Keywords:
gastrointestinal stromal tumor;
BMI1;
immunohistochemistry
- From:
Journal of Medical Postgraduates
2018;31(6):631-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective The role of BMI1 gene in the development of gastrointestinal stromal tumor (GIST) has not yet been clarified. This study aimed to explore the expression of BMI1 gene in gastrointestinal stromal tumor,and analyze its relationship with clinical pathological features of GIST. Methods The clinical data of 68 GIST patients treated in The First People's Hospital of Nan-ning from August 2012 to October 2015 were analyzed retrospectively. The expression of BMI1 in normal gastrointestinal tissues and GIST tissues were detected with immunohistochemistry method,and analyzed the relationship between various clinicopathological pa-rameters of GIST and BMI1. The expression of BMI1 protein was detected by Western blot. Results The positive rate of BMI1 was much higher in GIST group than in non-GIST (76.47% vs 36.84%,P<0.05). The difference in the expression of BMI1 protein between the different risk groups was statistically significant (P<0.05). The positive expression rate was the highest in the high-risk group (93.75%),but had no statistically significant difference among different genders,age,locations,histological types and whether me-tastasis (P>0.05). Expression of proliferation genes such as PCNA,CyclinD1 mRNA in BMI1 positive group were higher than those in BMI1 negative group,the expression of Pro-apoptotic genes such as Caspase-7,Smac mRNA were lower than those in BMI1 negative group,the expression of anti-apoptosis genes such as Livin,p53,Bcl-2 mRNA were higher than those in BMI1 negative group (P<0.05). Conclusion The expression of BMI1 protein was increased in GIST tissue. It is correlated with the risk classification,and is an important factor affecting the prognosis of patients.
