Effects of bacterial lipopolysaccharide on the mineralization and differentiation of periodontal ligament stem cells
10.16571/j.cnki.1008-8199.2018.06.003
- VernacularTitle:细菌脂多糖对牙周膜干细胞的矿化分化作用的影响
- Author:
Ting GUO
1
;
Gang CAO
;
Zhi LI
;
Wei CHEN
;
Xu LENG
;
Bing-Yao LIU
;
Jin-Ke XU
;
Zhao MAO
Author Information
1. 南京大学医学院附属口腔医院牙体牙髓病科
- Keywords:
periodontal tissue regeneration;
porphyromonas gingivalis lipopolysaccharide;
periodontal ligament stem cell;
mineralization;
differentiation
- From:
Journal of Medical Postgraduates
2018;31(6):573-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective Periodontal tissue engineering has shown a highlight prospect in the treatment of periodontitis,but the related clinical experiments have not achieved the predetermined goal. In this study,we analyzed the reasons for the limited clinical efficacy of periodontal tissue engineering. Methods We primarily cultured the periodontal tissue from the young permanent teeth extracted for or-thodontic treatment,isolated periodontal ligament stem cells (PDLSCs),and transplanted the well-grown third-generation PDLSCs onto the fluorapatite-polycaprolactone (FA-PCL) nanofiber scaffolds and PCL nanofiber scaffolds. We randomly divided the cells into groups A (cultured with 10 ng/mL porphyromonas gingivalis lipopo-lysaccharide (Pg-LPS)+FA-PCL),B (cultured with 10 ng/mL PG-LPS+PCL),C (cultured with 10 μg/mL PG-LPS+FA-PCL),D (cultured with 10 μg/mL PG-LPS+PCL),E (cultured with FA-PCL),and F (cultured with PCL),and observed their proliferation,differentiation and mineralization. Results The PDLSCs adhered and grew well after transplanted onto the nanofiber scaffolds and their proliferation significantly increased in groups A and B but decreased in C and D as compared with E and F. At 7 days,the expres-sions of ALP and mineralization-related genes runx2 and SPP1 in the PDLSCs were significantly higher in group E than in the other five groups (P<0.05),but higher groups A and C than in B and D as well as in A than in C. At 28 days,alizarin red and Von Kossa stai-ning showed a higher positivity in group E than in the other five groups,but higher groups A and C than in B and D as well as in A than in C. Conclusion The inflammatory environment not only affects the proliferation of PDLSCs,but also inhibits their differentiation and mineralization. The FA-PCL scaffold can reduce the cytotoxic effect of PG-LPS.
