Expressions of CaSR and Claudin-14 and the formation of nanobacterial renal stone: A dynamic study
10.16571/j.cnki.1008-8199.2018.05.004
- VernacularTitle:CaSR和Claudin-14在纳米细菌肾结石形成中表达的动态研究
- Author:
Mao-Lei SHEN
1
;
Qin-Zhang WANG
;
Cheng QIAN
;
Hao XU
;
Zhi-Qiang HAO
;
Peng LI
;
Biao QIAN
Author Information
1. 石河子大学医学院第一附属医院泌尿外科
- Keywords:
CaSR;
Claudin-14;
nanobacteria;
renal stone
- From:
Journal of Medical Postgraduates
2018;31(5):466-469
- CountryChina
- Language:Chinese
-
Abstract:
Objective Nanobacteria are one of the factors for urinary calculi and its exact pathogenic mechanism is not yet clear.The aim of this study was to investigate the role of the CaSR -Claudin-14 regulatory channel in the formation of calculi . Methods Sixty Wistar male rats were equally randomized into a normal control group and nanobacterial group , the former injected via the tail vein with 1.2 mL of 0.9％ sodium chloride solution while the latter with 1.2 mL of nanobacterial suspension , both for once.Three of the rats in each group were sacrificed every week in the first 10 weeks after injection.Histopathological examination was performed every week to evaluate the stone formation in the kidneys of the rats , and the expressions of the CaSR and Claudin -14 proteins were determined by immunohistochemistry. Results From the 1st to the 10th week after injection, crystal particles were observed in the rat kidneys of the nanobacterial group, but not in the normal controls (52.4％ vs 0％, P<0.01).The expressions of CaSR and Claudin -14 showed no statistically significant differences between the nanobacterial and control groups in the first 3 weeks (P>0.05) but both gradually in-creased in the former group from the 4th to the 10th week as compared with the latter, mainly in membrane of the renal tubular epithelial cells. Conclusion The increased activity of the CaSR -Claudin-14 regulatory channel may play an important role in the formation of nanobacterial renal stone .
