Upregulated expression of Caprin-1 increases the proliferation and migration of glioma U251 cells
10.16571/j.cnki.1008-8199.2018.01.003
- VernacularTitle:上调细胞周期相关蛋白-1的表达对脑胶质瘤U251细胞的增殖和迁移能力的影响
- Author:
Li-Dong YAN
1
;
Hui GUI
;
Zhuo-Shun YANG
;
Dan-Dan ZOU
;
Jue WANG
;
Li ZHANG
;
Kuan-Ming HUANG
;
Jie LUO
Author Information
1. 湖北医药学院附属医院(十堰市太和医院)神经外科一病区
- Keywords:
brain glioma;
U251 cell;
Caprin-1;
proliferation;
migration
- From:
Journal of Medical Postgraduates
2018;31(1):13-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective Cell cycle-associated protein 1 (Caprin-1) is closely related to the development and progression of cancer. This study aimed to explore the expression of Caprin-1 in the clinical glioma specimen and its influence on the biological char-acteristics of the glioma cell line. Methods Brain tissue specimens were collected from 29 glioma patients and 2 normal humans that died of accidental trauma. A stably transfected U251 cell line with overex-pressed Caprin-1 was established,and the U251 cells were transfected with the pEGFP-C1 plasmid (the negative control group), or the pEGFP-C1-Caprin-1 plasmid (the experimental group), or left un-transfected (the blank control group). The expressions of Caprin-1 mRNA and protein in the cells were determined by RT-PCR and Western blot, and the proliferation and migration of the cells exam-ined by scratch test and Transwell assay,respectively. Results The expression of Caprin-1 was upregulated with the increased grade of glioma,145.9±22.0,444.4±110.0,and 1661.0±54.5 in WHO gradeⅡ,Ⅲ,andⅣglioma,respectively,significantly higher than in the normal brain tissue (P<0.05). Both the mRNA and protein expressions of Caprin-1 were remarkably higher in the experimental group (1.70±0.19 and 1.07±0.09) than in the blank control(0.89±0.10 and 0.52±0.04) and negative control(0.98±0.08 and 0.58± 0.03) (P<0.05).The A value was also markedly higher in the former group(2.55±0.14) than in the latter two(1.40±0.06 and 1.35± 0.04) (P<0.01),and so were the count of migrated cells(526.00±42.19 vs 289.00±29.24 and 279.00±32.48,P<0.01) and the ex-pression of CyclinD1 (0.60±0.05 vs 0.13±0.03 and 0.15±0.05, P<0.01). Conclusion The expression of Caprin-1 in the U251 cells was upregulated with the increased WHO grade of glioma,and the overexpression of Caprin-1 accelerated the proliferation and mi-gration of the U251 cells.
