Effects of ApoC3 on the severity of acute pancreatitis in mice
10.3760/cma.j.issn.1674-1935.2018.02.011
- VernacularTitle:ApoC3基因高表达对小鼠急性胰腺炎严重程度的影响
- Author:
Yao YAO
1
;
Kun LIN
;
Lu ZHUANG
;
Xiaohua MA
;
Jing JIN
;
Hongyu WU
;
Yanfang GONG
;
Baoan JI
;
Haojie HUANG
;
Zhaoshen LI
Author Information
1. 第二军医大学长海医院消化内科
- Keywords:
Panceatitis;
Hyperlipidemias;
Apolipoproteins C
- From:
Chinese Journal of Pancreatology
2018;18(2):109-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of ApoC3 gene on the severity of hypertriglyceridemiainduced acute pancreatitis (AP).Methods ApoC3 transgenetic mice and C57BL/6J mice AP model was induced by cerulein intraperitoneal injection,and ApoC3 transgenetic mice and C57BL/6J mice injected by normal saline solution in equal volume served as control group.Serum triglyceride and cholesterol were detected,and the pathological changes of the pancreas were observed.RT PCR method was used to examine the changes of the inflammatory factor including IL-1β,IL-6,α-SMA and TNF-α mRNA levels,which reflected the severity of the inflammation.Results Serum triglyceride and cholesterol were higher in ApoC3 transgenetic mice than in C57BL/6J mice [(3.434 ± 0.931) mmol/L vs (0.766 ± 0.120) mmol/L,(2.553 ±0.178) mmol/L vs (1.996 ± 0.080) mmol/L],and the differences were statistically different (P ＜ 0.05).The pathological changes of the pancreas were more severe in ApoC3 transgenetic AP mice than in C57BL/6J AP mice,and the IL-1β,IL-6 and α-SMA mRNA levels in the pancreatic tissue were obviously higher in ApoC3 transgenetic AP mice than in C57BL/6J mice (1.72 ± 0.07vs 0.78 ± 0.09,1.58 ± 0.09vs 0.87 ±0.04,0.83 ± 0.05vs 0.44 ± 0.04),and the differences were statistically significant (P ＜ 0.05),while there was no statistical difference on TNF-αmRNA level (0.70 ± 0.09vs 0.65 ± 0.08,P ＞ 0.05).Conclusions ApoC3 gene could aggravate the severity of the inflammation in hypertriglyceridemia-induced AP.
