Lysimachia capillipes inhibits proliferation of pancreatic cancer BxPC-3 cells in vitro
10.3760/cma.j.issn.1674-1935.2018.02.008
- VernacularTitle:细梗香草皂苷对人胰腺癌BxPC-3细胞的体外抑制作用
- Author:
Xianjin YU
1
;
Liang ZHOU
;
Yahong HE
;
Xiaofeng ZHANG
Author Information
1. 浙江医院消化内科
- Keywords:
Pancreatic neoplasms;
Saponins;
Lysimachia capillipes;
Apoptosis
- From:
Chinese Journal of Pancreatology
2018;18(2):100-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of lysimachia capillipes(LC) on proliferation of human pancreatic cancer cell line BxPC-3 in vitro and explore the potential mechanism.Methods BxPC-3 cells were treated by LC in different concentrations of 2,4,8,16,32,64 μg/ml for 48 and 72 hours,respectively,using untreated cells as control.The survival rate of BxPC-3 cells was measured by MTT method.The half inhibition concentration (IC50) of LC was determined by drawing growth curve.BxPC-3 cells were treated by LC in the concentration of IC50 (LC group),and cell apoptosis and cell cycle were examined by using flow cytometry.The protein expression of PARP and capase-3 was detected by Western blotting.Results LC in the concentration of 8-32 μg/ml inhibited the survival rate of BxPC-3 cells in a dose-dependent manner.After exposure to 15 μg/ml LC for 48 h,the apoptosis rate of BxPC-3 cells was increased [(17.3 ± 0.31)％ vs (1.5 ± 0.22) ％],but the cell cycle was not affected.The expression of caspase-3 protein was up-regulated [(2207.2 ± 92.0) vs 149.1 ± 10.2] and PARP protein was down-regulated [(36.1 ± 4.8) vs 1593.4 ±29.7] than control group,and the differences were statistically different (all P value ＜ 0.05).Conclusions LC can inhibit the growth of BxPC-3 cells,and the potential mechanism was associated with the induction of cell apoptosis by LC via upregulating caspase-3 protein expression and decompsing PARP protein.
