Effects of oxidative stress on the activation of pancreatic stellate cells through the up-regulation of fibromodulin
10.3760/cma.j.issn.1674-1935.2017.06.004
- VernacularTitle:纤调蛋白对氧化应激诱导的胰腺星形细胞活化的影响
- Author:
Jianwei ZHU
1
;
Huiyun ZHU
;
Fei JIANG
;
Wei AN
Author Information
1. 第二军医大学长海医院消化内科
- Keywords:
Pancreatitis,chronic;
Pancreatic stellate cells;
Oxidative stress;
Fibromodulin
- From:
Chinese Journal of Pancreatology
2017;17(6):371-374
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of fibromodulin (FMOD) in the xidative stress induced activation of pancreatic stellate cells (PSCs).Methods Lentivirus containing ShRNA targeting FMOD (sh-FMOD) was transfected into PSCs,and then the prooxidant menadione (MND) was used to treat PSCs for 24 h (MND + sh-FMOD group).Lentivirus transfected PSCs cells treated by a equal volume of DMSO served as shFMOD group,parent cells as control group and PSCs treated by MND as MND group.RT-PCR were used to detect the mRNA expression of the markers of activated PSCs including α-SMA,Col3α1,Col1α1,TIMP1 and α1-integrin.Chronic pancreatitis (CP) rat model was induced by DBTC infusion into the tail vein.Immunohistochemical (IHC) staining was used to detect the protein expression of FMOD,FN,α-SMA,SOD and MDA in normal pancreatic tissue and CP tissue.Results FMOD mRNA expression of the PSCs in FMOD group was obviously lowerer than that in control group (0.16 ±0.03 vs 1),and the difference was statistically significant (P ＜ 0.01),indicating that FMOD was successfully silenced.The mRNA expression of FMOD,α-SMA,Col3α1,Col1α1,TIMP1 and α1-Integrin of PSCs in MND group was obviously higher than those in control group,which in MND + sh-FMOD group was lower than those in MND group,and the difference was statistically significant (P ＜0.05 or ＜0.01).Compared with those in normal pancreatic tissue,the protein expression of FMOD,α-SMA,SOD and MDA in CP tissue was up-regulated,and the difference was statistically significant (all P ＜ 0.05).Conclusions Oxidative stress can facilitate the activation of PSCs through the induction of fibromodulin expression.
