Mass spectrometry detection of basic drugs in fast chiral analyses with vancomycin stationary phases
- Author:
Hongyue GUO
1
;
Wahab Farooq M.
;
Berthod ALAIN
;
Armstrong W. DANIEL
Author Information
1. Department of Chemistry and Biochemistry
- Keywords:
Mass spectrometry detection;
Chiral separation;
Basic drugs;
Beta-blockers;
Superficially porous particle;
Fully porous particle;
Fast separation;
Vancomycin
- From:
Journal of Pharmaceutical Analysis
2018;8(5):324-332
- CountryChina
- Language:Chinese
-
Abstract:
Current trends in chiral analysis of pharmaceutical drugs are focused on faster separations and higher separation efficiencies. Core-shell or superficially porous particles (SPP) based chiral stationary phases (CSPs) provide reduced analysis times while maintaining high column efficiencies and sensitivity. In this study, mobile phase conditions suitable for chiral analyses with electrospray ionization LC-MS were systematically investigated using vancomycin as a representative CSP. The performance of a 2.7 μm SPP based vancomycin CSP (SPP-V) 10 cm × 0.21 cm column was compared to that of a corresponding 5 μm fully porous particles based analogue column. The results demonstrated that the SPP-V column provides higher efficiencies, 2–5 time greater sensitivity and shorter analysis time for a set of 22 basic pharma-ceutical drugs. The SPP-V was successfully applied for the analysis of the degradation products of racemic citalopram whose enantiomers could be selectively identified by MS.
