Eco-friendly reduced graphene oxide for the determination of mycophenolate mofetil in pharmaceutical formulations
- Author:
Narayan S. PRASHANTH
1
;
Teradal L. NAGAPPA
;
Jaldappagari SEETHARAMAPPA
;
Satpati K. ASHIS
Author Information
1. Department of Chemistry
- Keywords:
Mycophenolate mofetil;
Electroreduced graphene oxide;
Electrochemical sensor;
Analytical application
- From:
Journal of Pharmaceutical Analysis
2018;8(2):131-137
- CountryChina
- Language:Chinese
-
Abstract:
Graphene oxide (GO) was synthesized and characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), atomic force microscopy (AFM), X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA). GO was then electro-chemically reduced and used for electrochemical study of mycophenolate mofetil (MMF). The electro-chemically reduced graphene oxide (ERGO) film on glassy carbon electrode (GCE) showed enhanced peak current for electrooxidation of MMF. MMF exhibited two irreversible oxidation peaks at 0.84 V (peak a1) and 1.1 V (peak a2). Effects of accumulation time, pH and scan rate were studied and various electro-chemical parameters were calculated. A differential pulse voltammetric method was developed for the determination of MMF in bulk samples and pharmaceutical formulations. Linear relationship was ob-served between the peak current and concentration of MMF in the range of 40 nM―15μM with a limit of detection of 11.3 nM. The proposed method is simple, sensitive and inexpensive and, hence, could be readily adopted in clinical and quality control laboratories.
