Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry
- Author:
Janssens YORICK
1
;
Verbeke FREDERICK
;
Debunne NATHAN
;
Wynendaele EVELIEN
;
Peremans KATHELIJNE
;
Spiegeleer De BART
Author Information
1. Drug Quality and Registration (DruQuaR) Group
- Keywords:
Peptide iodination;
LC–UV/MS;
Quorum sensing;
Bolton-Hunter
- From:
Journal of Pharmaceutical Analysis
2018;8(1):69-74
- CountryChina
- Language:Chinese
-
Abstract:
Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quan-tification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono-and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. De-pending on the used iodination method, iodination yields varied from low (2%) to high (57%).
