Anti-herpes Activity of Vinegar-processed Daphne genkwa Flos Via Enhancement of Natural Killer Cell Activity.
- Author:
Erdenebileg UYANGAA
1
;
Jin Young CHOI
;
Hyung Won RYU
;
Sei Ryang OH
;
Seong Kug EO
Author Information
- Publication Type:Original Article
- Keywords: Daphne genkwa; Vinegar-processed flos; Herpes simplex virus; Genital infection; NK cells
- MeSH: Animals; Daphne*; Herpesvirus 1, Human; Inflammation; Interferons; Killer Cells, Natural*; Mice; Mortality; Simplexvirus; Therapeutic Irrigation; Ulcer
- From:Immune Network 2015;15(2):91-99
- CountryRepublic of Korea
- Language:English
- Abstract: Herpes simplex virus (HSV) is a common causative agent of genital ulceration and can lead to subsequent neurological disease in some cases. Here, using a genital infection model, we tested the efficacy of vinegar-processed flos of Daphne genkwa (vp-genkwa) to modulate vaginal inflammation caused by HSV-1 infection. Our data revealed that treatment with optimal doses of vp-genkwa after, but not before, HSV-1 infection provided enhanced resistance against HSV-1 infection, as corroborated by reduced mortality and clinical signs. Consistent with these results, treatment with vp-genkwa after HSV-1 infection reduced viral replication in the vaginal tract. Furthermore, somewhat intriguingly, treatment of vp-genkwa after HSV-1 infection increased the frequency and absolute number of CD3-NK1.1+NKp46+ natural killer (NK) cells producing interferon (IFN)-gamma and granyzme B, which indicates that vp-genkwa treatment induces the activation of NK cells. Supportively, secreted IFN-gamma was detected at an increased level in vaginal lavages of mice treated with vp-genkwa after HSV-1 infection. These results indicate that enhanced resistance to HSV-1 infection by treatment with vp-genkwa is associated with NK cell activation. Therefore, our data provide a valuable insight into the use of vp-genkwa to control clinical severity in HSV infection through NK cell activation.
