1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) Modulates Th2 Immunity through Attenuation of IL-4 Expression.
- Author:
Sun Young YOON
1
;
Ho Bum KANG
;
Young Eun KO
;
Su Hyun SHIN
;
Young Jun KIM
;
Ki Young SOHN
;
Yong Hae HAN
;
Saeho CHONG
;
Jae Wha KIM
Author Information
- Publication Type:Original Article
- Keywords: EC-18; Th2 modulator; IL-4; STAT6
- MeSH: Animals; Cell Line; Dermatitis, Atopic; Hypersensitivity; Interleukin-4*; Mice; Phenotype; Phosphorylation; STAT6 Transcription Factor
- From:Immune Network 2015;15(2):100-109
- CountryRepublic of Korea
- Language:English
- Abstract: Controlling balance between T-helper type 1 (Th1) and T-helper type 2 (Th2) plays a pivotal role in maintaining the biological rhythm of Th1/Th2 and circumventing diseases caused by Th1/Th2 imbalance. Interleukin 4 (IL-4) is a Th2-type cytokine and often associated with hypersensitivity-related diseases such as atopic dermatitis and allergies when overexpressed. In this study, we have tried to elucidate the function of 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) as an essential modulator of Th1/Th2 balance. EC-18 has showed an inhibitory effect on the production of IL-4 in a dose-dependent manner. RT-PCR analysis has proved EC-18 affect the transcription of IL-4. By analyzing the phosphorylation status of Signal transducer and activator of transcription 6 (STAT6), which is a transcriptional activator of IL-4 expression, we discovered that EC-18 induced the decrease of STAT6 activity in several stimulated cell lines, which was also showed in STAT6 reporter analysis. Co-treatment of EC-18 significantly weakened atopy-like phenotypes in mice treated with an allergen. Collectively, our results suggest that EC-18 is a potent Th2 modulating factor by regulating the transcription of IL-4 via STAT6 modulation, and could be developed for immune-modulatory therapeutics.
