Expression and correlation of Toll-like receptor 2, LC3B and Beclin1 in glioma
10.3760/cma.j.issn.1673-4904.2018.10.008
- VernacularTitle:胶质瘤中Toll样受体2与LC3B、Beclin1的表达变化及相关性研究
- Author:
Lixin MA
1
,
2
;
Zhihui LIU
;
Yongliang LIU
;
Qingbo WANG
;
Zefu LI
;
Chenglong LI
Author Information
1. 256603 滨州医学院附属医院神经外科
2. 首都医科大学三博脑科医院,北京100093
- Keywords:
Glioma;
Toll-like receptor 2;
Autophagy
- From:
Chinese Journal of Postgraduates of Medicine
2018;41(10):896-900
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between Toll-like receptor 2(TLR2) and autophagy marker protein in glioma. Methods Glioma tumors of a total of 74 patients from June 2012 to December 2017 were surgically resected, including WHO gradeⅠtoⅡ32 cases, grade Ⅲ 20 cases, gradeⅣ22 cases. Immunohistochemistry and Western blot were used to detect the expression of TLR2, autophagy related protein LC3B, Beclin 1 and apoptosis related protein Bax and Bcl-2. The correlation between TLR2 and autophagy related protein LC3B and Beclin 1 were analyzed. Results In high grade glioma (HGG) tissue and low grade glioma (LGG) tissue, the TLR2 positive expression rates were 92.9％(39/42) and 75.0％(24/32), and there was significant difference (P<0.05). In HGG tissue, autophagy related protein LC3B, Beclin1 protein was strongly positive and the positive expression rates were 45.2％(19/42) and 52.4％(22/42). In LGG tissue, LC3B and Beclin1 protein positive expression rates were 18.8％(6/32) and 15.6％(5/32), and there were significant differences (P<0.05). Spearman correlation analysis showed that TLR2 protein was closely related to autophagy related protein LC3B (r=0.5638, P<0.05) and Beclin1 (r=0.6101, P<0.05). Conclusions TLR2 is highly expressed in HGG tissue, and its expression level may be related to autophagy, which has potential value as a targeted therapy.
