Study on expression of the guanylate binding protein 5 and ArfGAP with SH3 domain ankyrin repeat and PH domain 1 genes among pulmonary tuberculosis patients and populations with latent tuberculosis infection
10.3760/cma.j.issn.1673-4904.2018.06.002
- VernacularTitle:鸟苷酸结合蛋白5和二磷酸腺苷核糖化因子鸟苷酸激酶1基因在肺结核患者和潜伏结核感染人群中的表达研究
- Author:
Weiwei GONG
1
,
2
;
Jinfeng LI
;
Aihua SUI
;
Xinhong ZHU
;
Cunzhi LIN
Author Information
1. 266003 青岛大学附属医院呼吸内科
2. 山东省枣庄市王开传染病医院内三科, 277500
- Keywords:
Tuberculosis,pulmonary;
Latent tuberculosis infection;
Guanylate binding protein 5;
ArfGAP with SH3 domain ankyrin repeat and PH domain 1;
Gene expression
- From:
Chinese Journal of Postgraduates of Medicine
2018;41(6):485-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of guanylate binding protein 5 (GBP5) and ArfGAP with SH3 domain ankyrin repeat and PH domain 1 (ASAP1) genes in pulmonary tuberculosis patients and tuberculosis latent population. Methods Forty pulmonary tuberculosis patients (pulmonary tuberculosis group), 40 latent tuberculosis infection patients (latent tuberculosis infection group) and 40 cases of healthy control (healthy control group) were selected from August 2016 to May 2017. The gene expression was detected in 4 ml peripheral anticoagulant blood by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) and the relative expression of two genes in three groups were compared. Results The GBP5 gene expression in three groups was significantly differentce (F=7.23, P=0.001). The GBP5 gene relative expression in pulmonary tuberculosis group was significantly higher than that in latent infection group :1.58 ± 0.80 vs. 1.09 ± 0.68, there was significant difference (t=2.93, P=0.004). The GBP5 gene relative expression in pulmonary tuberculosis group was significantly higher than that in healthy control group: 1.58 ± 0.80 vs. 1.04 ± 0.61, there was significant difference (t=3.40, P=0.010). The GBP5 gene relative expression in latent infection group and healthy control group had no significant difference (t=0.39, P=0.700). There was no significant difference in ASAP1 expression among three groups (F=0.26, P=0.770). Conclusions The expression of GBP5 in pulmonary tuberculosis patients, latent tuberculosis infection patients and healthy controls is different, and GBP5 could screen latent tuberculosis infection patients which is expected to be a potential screening marker for latent tuberculosis infection.
